EFFECT OF SIMPLE CLEANSING METHODS ON MOUTHPIECES DECONTAMINATION OF SHARED WIND INSTRUMENTS
Sri Agung Fitri Kusuma*, Wahyu Purwita Sari and Dewi Rusmiati
ABSTRACT
Objective: This study was designed to determine a simple cleansing
method that can be applied to decontaminate mouthpieces of shared
wind instruments. Methods: In this study, 4 kinds of wind instruments,
i.e. trumpet, mellophone, trombone and tuba, were examined. The total
bacteria attached to the instrument was isolated using the swab method
and suspended it in a sterile physiological NaCl solution to determine
the number of initial bacterial colonies on the instrument. The effect of
decontamination was carried out by a simple cleansing method using
immersion the mouthpieces in hot water with the temperature of 100
0C for 5 min and soaked using soap containing 2% of triclosan. The
survival colonies were evaluated using plate count method and
calculated using a colony counter. Results: The cleansing method using immersion in hot
water with the temperature of 100 0C for 5 min significantly decreasing the bacteria number
(91.85-99.91%), compared with that of liquid soap (50.30-91.67%). However, tuba
mouthpieces were the most easiest to be sterilized using both methods, because it has the
largest surface area. Conclusion: The immersion using hot water with the temperature of 100
0C for 5 min can be used as a method of disinfection that is fast, simple and easy to be
applied to the mouthpieces of shared wind instruments.
Keywords: disinfection, mouthpieces, musical instruments, hot water, soap, simple.
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