Abstract

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHODS FOR SIMULTANEOUS ESTIMATION OF CEFUROXIME AXETIL AND LINEZOLID IN PHARMACEUTICAL DOSAGE FORM

M. R. Sakhare, P. S. Jirvankar, S. K. Rabade, Dr. M. J. Umekar and R. T. Lohiya*

ABSTRACT

In the present work two simple, accurate, sensitive, precise and economical UV spectrophotometric methods (Method-A and Method- B) have been developed for the simultaneous estimation of Cefuroxime axetil and Linezolid in pharmaceutical dosage form. Method-A is Area Under Curve Spectrophotometry, the wavelength range selected for quantitation are 271.4 – 281.4 nm and 252.4 – 262.4 nm. Whereas Method-B is Multicomponent Mode Analysis, Wavelength selected were 276.4 nm (λmax of CEF) and 257.4 nm (λmax of LIN) and 272.4 nm (isobestic point) for the analysis. Both the methods were found linear between 5-25 μg/mL for cefuroxime axetil and 6-30 μg/mL for linezolid. The methods showed good reproducibility and recovery with % RSD less than prescribed limit. Methods were successfully applied for the simultaneous determination of both the drugs in commercial tablet formulation. The results of the analysis have been validated statistically and by recovery studies. So it can be used for routine analysis of both drugs in quality control laboratories.

Keywords: Cefuroxime axetil (CEF), Linezolid (LIN), Area Under Curve Method, Multicomponent Mode of Analysis.