Abstract

ANTI-COLITIC ACTION OF METHANOL EXTRACT OF MUSA SAPIENTUM (BANANA) PEELS ON ACETIC ACID INDUCED COLITIS IN DIABETIC RATS

Austin Azubuike Ajah, Tosan Peter Omayone*, Adeoti Gbemisola Adegoke and Samuel Adetunji Onasanwo

ABSTRACT

Diabetes mellitus and ulcerative colitis are frequent comorbid condition. Management of colitis can be very challenging in diabetic patients because of the need to control blood glucose levels. It is therefore thoughtful, that an agent with both anti-inflammatory and hypoglycaemic properties may be helpful. Musa sapientum has demonstrated both anti-inflammatory and hypoglycaemic potentials. In this study, the effect of methanolic extract of Musa sapientum peel (MEMSP) was investigated in acetic acid induced colitis in diabetic rats. A total of 50 male Wistar rats were divided into 5 groups (n=10). Group I served as colitis control with no diabetes. Animals in group IIV were induced with diabetes by the administration of streptozotocin (70mg/kg) and 72 hours later were induced with colitis by intrarectal instillation of 2ml, 6% (v/v) acetic acid. Groups I and II received distilled water, groups III and IV received MEMSP at a dose of 100 and 200 mg/kg respectively, while group V received sulfasalazine (50 mg/kg). Treatments commenced 24 hours following colitis induction. Fasting blood sugar level, relative colon weight, macroscopic and microscopic score of colon mucosa were accessed at 7- and 14- days post colitis induction to determine disease severity. Relative colon weight, Macroscopic and microscopic sores were significantly increased in dia+colitis group compared to colitis alone group, while both concentrations of MEMS peel and sulfasalazine significantly reduced these variables compared to dia+col group. The results suggest that MEMS peel and sulfasalazine may be effective in the treatment of ulcerative colitis in diabetic condition.

Keywords: Comorbidity, Ulcerative colitis, Diabetes, Musa sapientum, Strecptozotocin, Acetic acid.