IN VITRO STUDY OF PETROLEUM ETHER EXTRACT OF ECLIPTA ALBA Hassk. FOR HepG2 CELL LINE
Dr. Tathe Mangal Suresh*, Dr. Kulkarni D. V. and Dr. Harke Sanjay Ningappa
ABSTRACT
With advanced technologies we can find new bridge between
Ayurveda and modern researches, for that we tried here petroleum
ether extract of Eclipta alba against hepatic cancer. Due to having
potent anti-cancer activity in Eclipta alba, it can be successfully used
in cancer management both as mainline treatment and an adjuvant
medication with minimum side effects. With the help of this paper the
Eclipta alba which will prove to be helpful for the researcher
development clinical trial and it can be used in the clinical practices for
treating hepatic cancer in near future. Future research on such a topic
would help to identify safe, pure and effective anticancer drugs and
will further the discovery of their mechanism of action. We have used petroleum ether extract
of Eclipta alba Hassk. for phytochemical analysis, TLC, HPLC analysis to test active
chemical components in it. All tests proved that an petroleum ether extract of Eclipta alba
Hassk. containing many active chemical components, therefore our experiment showed the
positive results for an petroleum ether extract of Eclipta alba Hassk. against hepatic cancer.
The srb assay results were used to evaluate the anti-cancer activity of the extract. The effects
of whole plant extract on cancer cell line were studied and evaluated. The percentage of cell
growth and cell viability were calculated from tabulated result values of srb assay. The
experiment revealed that the average percentage of growth inhibition was 55.06%. Cell
viability srb assay also showed significant growth inhibition, at the same time statistical analysis of srb assay also proved significant results. The research performed here is very useful for setting up of different extract studies of Bhringraj for its anticancer activity.
Keywords: Eclipta alba Hassk., HepG2, petroleum ether extract, (Sulforhodamine B) srb assay.
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