Abstract

PHYTOCHEMICAL, PHARMACOGNOSTICAL AND QUANTITATIVE ESTIMATION OF PONGAMIA PINNATA LEAVES EXTRACT-A PRELIMINARY STUDY TO IDENTIFIED PHYTOCONSTITUENTS

S. K. Sarje*, Shagufta Farooqui, Shinde Punam, Sontakke Shital and Narote Mayur

ABSTRACT

Objective: The objective of this study was to carry out phytochemical and pharmacognostic and quantitative evaluation of leaves of Pongamia pinnata L. (Fabacea). Method: The present study provides pharmacognostic, phytochemical and quantitative details of the leaves of P. pinnata. Results: The macroscopic study showed that the leaf was ovate or elliptic with smooth margins, short petiole, alternate imparipinnate, hairless, acuminate at apex, rounded to cuneate at base and slightly thickened. Microscopic study revealed collateral, closed vascular bundles, trichomes, paracytic stomata, xylem vessels and prismatic calcium oxalate crystals. Qualitative Phytochemical screening showed the presence of alkaloids, glycosides, carbohydrates, steroids and flavonoids and phenolic compounds in both the extracts. Total Poly phenol content & total flavonoid content was determined by Folin Ciocaltue & Aluminium trichloride method respectively by using UV-Visible spectrophotometer. DPPH scavenging assay were performed to evaluate the antioxidant activity which was found maximum at 125 μg/ml concentration for both the extracts. Conclusions: The results of this study can serve as valuable source of information for identification of this plant for future investigation and applications.

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