PHYTOCHEMICAL, PHARMACOGNOSTICAL AND QUANTITATIVE ESTIMATION OF PONGAMIA PINNATA LEAVES EXTRACT-A PRELIMINARY STUDY TO IDENTIFIED PHYTOCONSTITUENTS
S. K. Sarje*, Shagufta Farooqui, Shinde Punam, Sontakke Shital and Narote Mayur
ABSTRACT
Objective: The objective of this study was to carry out phytochemical
and pharmacognostic and quantitative evaluation of leaves of
Pongamia pinnata L. (Fabacea). Method: The present study provides
pharmacognostic, phytochemical and quantitative details of the leaves
of P. pinnata. Results: The macroscopic study showed that the leaf
was ovate or elliptic with smooth margins, short petiole, alternate
imparipinnate, hairless, acuminate at apex, rounded to cuneate at base
and slightly thickened. Microscopic study revealed collateral, closed
vascular bundles, trichomes, paracytic stomata, xylem vessels and
prismatic calcium oxalate crystals. Qualitative Phytochemical
screening showed the presence of alkaloids, glycosides, carbohydrates, steroids and
flavonoids and phenolic compounds in both the extracts. Total Poly phenol content & total
flavonoid content was determined by Folin Ciocaltue & Aluminium trichloride method
respectively by using UV-Visible spectrophotometer. DPPH scavenging assay were
performed to evaluate the antioxidant activity which was found maximum at 125 μg/ml
concentration for both the extracts. Conclusions: The results of this study can serve as
valuable source of information for identification of this plant for future investigation and
applications.
Keywords: .
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