Abstract

A SIMPLE SPECTROPHOTOMETRIC QUANTITATIVE DETERMINATION OF ACENOCOUMAROL IN BULK AND PHARMACEUTICAL DOSAGE FORMS USING DNPH REAGENT

G. Sreedevi*, D. Venkateswarlu, I.E. Chakravarthy, N. Rami Reddy, K. Prabhavathi

ABSTRACT

A simple, sensitive, cost effective spectrophotometric method developed and validated for the determination of Acenocoumarol, in bulk drug and its pharmaceutical formulations. The method is rely on the formation of a hydrazone with 2,4-dinitrophenylhydrazine (DNPH); the reaction of drug with reagent gives a bright yellow colour. The obtained coloured species absorbance was measured at its absorption maximum (λmax) 486 nm. The Beer’s law has been obeyed in the concentration range 50-250 μg/ml. All the variables were examined to optimize the reaction conditions. There was no interference observed in the presence of common pharmaceutical excipients. The validity of the method was established by analyzing CIL in its pharmaceutical formulations and critically tested for its accuracy by statistical tests. Good recoveries were obtained by the developed method; the obtained results were critically analyzed and successfully employed for the determination of Acenocoumarol in its pharmaceutical dosage forms.

Keywords: Acenocoumarol, DNPH, Spectrophotometry, antiplatelet- agregation, Vali.