A SIMPLE SPECTROPHOTOMETRIC QUANTITATIVE DETERMINATION OF ACENOCOUMAROL IN BULK AND PHARMACEUTICAL DOSAGE FORMS USING DNPH REAGENT
G. Sreedevi*, D. Venkateswarlu, I.E. Chakravarthy, N. Rami Reddy, K. Prabhavathi
ABSTRACT
A simple, sensitive, cost effective spectrophotometric method
developed and validated for the determination of Acenocoumarol, in
bulk drug and its pharmaceutical formulations. The method is rely on
the formation of a hydrazone with 2,4-dinitrophenylhydrazine
(DNPH); the reaction of drug with reagent gives a bright yellow
colour. The obtained coloured species absorbance was measured at its
absorption maximum (λmax) 486 nm. The Beer’s law has been obeyed
in the concentration range 50-250 μg/ml. All the variables were
examined to optimize the reaction conditions. There was no
interference observed in the presence of common pharmaceutical excipients. The validity of
the method was established by analyzing CIL in its pharmaceutical formulations and
critically tested for its accuracy by statistical tests. Good recoveries were obtained by the
developed method; the obtained results were critically analyzed and successfully employed
for the determination of Acenocoumarol in its pharmaceutical dosage forms.
Keywords: Acenocoumarol, DNPH, Spectrophotometry, antiplatelet- agregation, Vali.
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