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Archana Rajaram Pawar*, Pratiksha D. Khade and Sagar K. Sabale


Infrared (IR) spectroscopy is a vibrational spectroscopic technique which is based on the absorption of infrared radiation by matters which results into excitation of vibration of molecular band. It is a powerful as advantageous method for investigating functional, structural, and compositional changes in cells, biomolecules, and tissues. In the recent years, (vibrational spectroscopies) infrared spectroscopy have been developed for all types of analysis in microbiology. Important characteristics of these methods are the relative ease with which measurements can be performed. Fourier transform infrared spectroscopy (FT-IR) is a technique that has been used over the years for the identification of substances in chemical analysis and is one that may be applied to the characterization of microorganisms. This paper intends to review the basic theory of Infrared Spectroscopy, Principle, Instrumentation, Sampling Techniques and its applications in the field of Analytical Science. It can be concluded that vibrational spectroscopy shows high potential as novel methods.

Keywords: Infrared spectroscopy, Near Infrared, Mid Infrared, Far Infrared, molecular vibrations, Fourier Transform spectrophotometers, Instruments, Sampling Techniques, Detector.

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