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Abstract

INVITRO ANTI-UROLITHIATIC ACTIVITY OF ALPINIA GALANGA ROOT EXTRACT

Sanakattula Sreevani*, S. Kanijasree, K. S. Sara Bhanu, S. Pushpa Latha, M. Haritha, O. Lakshmi Prasanna and S. Hussain Thaharun

ABSTRACT

The present study have been designed to evaluate the Invitro Antiurolithiatic activity of herbal plant Alpinia galangal belonging to the family zingiberaceae. The dried powder of roots were collected and extracted by Soxhlet with solvents like methanol and chloroform. To investigate the inhibitory effect of extract on in invitro crystals through analysing aggregation assay. Materials and methods: Aqueous extraction of Alpinia galanga powder was prepared and arranged in the different concentrations. Homogenous precipitation methods was used to prepare artificial stones such as calcium oxalate and semi-permeable membrane of egg was used as dissolution bags. Dissolution model were incubated in 72hrs and after that, the entire content in dissolution bags was estimated spectrophotometrically. The inhibitory activity of Alpinia galangal powder was extracted on the aggregation assay of calcium oxalate crystals was determined by spectrophotometric assay. The percentage of Anti-urolithiatic activity is obtained as 74%,75%,76%,78%,80% for chloroform extract and 74%,75%,77%,78%,79% for methanol extract respectively at a dose of 50 mg/ml,100 mg/ml, 150 mg/ml, 200 mg/ml, 250, mg/ml. The percentage of Standard Neeri was found out to be 81%,82%,83%,84%,85% respectively at a dose of 50mg/ml, 100mg/ml, 150mg/ml, 200mg/ml, 250mg/ml. chloroform extract was more effective than methanol extract.

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