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Abstract

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF BACTERIA FROM COINS COLLECTED FROM DIFFERENT SOURCES

Sambuddha Ghosh, Saswati Mandal, Shalmali Chatterjee, Kumara Swamy Shreelakshmi and Shruthi Shirur Dakappa*

ABSTRACT

Money is acting as a potential vehicle in the transfer of a various diseases, as it is being handled by people of various classes. Thus, this study was conducted to highlight the need for proper hygiene in order to avoid diseases that could arise due to uncleansed hands. In this study, coins were collected from 10 different sources: restaurant, street vendor, bakery, tea seller, fruit seller, butcher’s shop, grocery, xerox shop, stationary shop and medicine shop. Collected coins were processed for the presence of cultivable bacteria. Identification of bacterial isolates was performed by culturing and biochemical investigations. Characterization was done by sequencing 16SrRNA gene. Results were found to bear major pathogenic organisms like Escherichia coli, Pseudomonas stutzeri, Bacillus cereus, Salmonella enterica etc. It witnessed visible presence of pathogens responsible for gastrointestinal infections in coins from places where people consume food while simultaneously handle money. The chances of cross contamination increases and in developing countries like India, poor hygiene practices after or while handling currency is the main reason behind maximum of the population suffering from stomach, nosocomial infections or any other forms of infection.

Keywords: coins, contamination, money, pathogenic microorganisms, characterization.


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