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Abstract

GENETIC DIVERSITY ANALYSIS OF MILLET CROP SPECIES OF PANICUM GENUS USING RAPD MARKERS

Aarti Desai and Shruthi Shirur Dakappa*

ABSTRACT

Millets are a group of diversified crops and highly nutritional. Random amplified polymorphic DNA (RAPD) was evaluated as a source of genetic markers for studying variation among species of Panicum. Screening of crop primers with 7 genotypes of millet crops species belonging to various genus was done. Four primers OPC06, OPC18, OPD13 and OPW04 were selected for PCR based analysis of these RAPD markers. Gel electrophoresis documentation was used for scoring of binary matrices by software PyElph 1.4. Further, the binary scores were analyzed by PAST 325 software to construct the dendrogram based on Euclidean distance. The primers OPC06 and OPD13 showed the highest polymorphism (83%) and maximum scoring of bands based on which phylogeny was established between the crop variants of Panicum species and the wild natal buffalo grass. Wild grass roots from the different clade and distant relative of millet crops of Panicum genus, showing its diverse genetic makeup. Natal buffalo grass is considered as millet but not included in crops and cereals, because of the evolutionary changes and environmental conditions.

Keywords: genetic diversity, polymorphism index, RAPD markers.


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