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Abstract

UV SPECTROPHOTOMETRIC AREA UNDER CURVE METHOD FOR THE SIMULTANEOUS DETERMINATION OF BISOPROLOL FUMARATE AND CILNIDIPINE IN PHARMACEUTICAL DOSAGE FORM

Shubhada Pawar*, Ashpak Tamboli and Snehal Patil

ABSTRACT

It is new precise, rapid, accurate UV spectroscopic method. In that area under curve method involved measurement of integrated area between selected wavelength ranges. For measurement of area wavelength range was found to be 222.20 -232.40nm (ʎ1-ʎ2) and 231-254.80nm (ʎ3-ʎ4) for Bisoprolol Fumarate and Cilnidipine respectively. UV Spectroscopic Area under Curve method was performed by using methanol: water (80:20) as a solvent system in Bulk as well as pharmaceutical dosage form. For that analyzed method linearity range were found to be 5-15μg/ml & 10-30μg/ml for Bisoprolol Fumarate and Cilnidipine respectively and it has regression coefficient was found to be 0.999 for both drug. This proposed method according to ICH guideline.

Keywords: Bisoprolol Fumarate, Cilnidipine, Area under Curve method and Validation.


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