Abstract

BIOFABRICATION OF SILVER SULPHIDE NANOPARTICLES FROM CINNAMOMUM TAMALA LEAVES: A NEXT GENERATION ANTIINFLAMMATORY AGENT

Richa Kothari* and Deepshikha Sharma

ABSTRACT

Objectives: Inflammation is a body response to fight against various infections, toxins and injuries. The inflammation could be acute or chronic. A few factors such as age, obesity, diet, low sex hormones, sleep disorders, cardiovascular disease and cancer are the root cause of inflammation. The objective of the present study was to biosynthesize nanoparticles with potential anti-inflammatory activity against standard acetylsalicylic acid (aspirin drug). Materials and Methods: Silver sulphide nanoparticles were fabricated using the sol-gel method by mixing of bay leaf extract in silver nitrate solution. The synthesised Ag 2 SNPs were then later characterised using UV-Visible spectroscopy,
FT-IR, SEM, TEM and XRD. Further proceed for their valuation of anti-inflammatory activity through protein- denaturation, protease inhibition assay and heat induced haemolysis method. Result: The various bio physical characterization proved that the particle size was below 100nm and of spherical shape. FTIR spectra analysis showed the presence of various biomolecules, which have a very significant role in capping and stabilising silver sulphide nanoparticles. The synthesised Ag2 S NPs showed anti- inflammatory activity against standard drug acetylsalicylic acid. Using protein denaturation, protease inhibition assay and heat induced haemolysis method. Conclusion: The biosynthesised silver sulphide nano particles could be used in several biomedical applications. The proposed synthesis is cost effective, eco-friendly and promising candidate for formulation of various topical applications like formulation of ointment, gel etc.

Keywords: Bio fabrication, Nanostructure, Spectroscopic characterization, Anti –inflammatory activity.