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Santosh M. Kurbetti*, Dr. Rakesh Kumar Jat and Dr. Sachin S. Mali


The aim of this study was to evaluate the antimicrobial effects of Ethanol, Aqueous, Chloroform & Methanol solvent against human pathogenic bacteria like Bacillus cereus, Staphylocous aureus, Escherichia coli, Pseudomonas aeruginosa & was determined by in vitro agar diffusion method. The herbal plant Piper Cubeba Leaves is selected and was evaluated for its phytochemical constituents, physicochemical property & antimicrobial activity. The extract was prepared by maceration extraction process by using aqueous solvent, Chloroform solvent & Ethanol solvent & also by Continuous hot extraction-Soxhelation process by using Methanol solvent. The extracts was formulated in different concentration of 1%, 2%, 3% & 4% by using same solvent & compared with the standard antibiotic like Amoxicillin (10μg/ml). Agar well diffusion method was used and the extract showed extensive zones of inhibition beside the tested isolates. Better antimicrobial activities were obtained with extract which gave better minimum inhibitory concentration (MIC) for the selected .In this study it has been showed that the 4% Ethanol extract of Piper Cubeba Leaves (E.E.P.C.L.) which was prepared by hot extraction process (soxhelation) had wider range of antimicrobial activity on used organisms than the aqueous, chloroform & methanol extract by maceration extraction process. This indicates that the Piper Cubeba leaves which are extracted by ethanol solvent using soxhelation process may contain more active components. This study supports the traditional medicines (herbal extracts) to cure many diseases like Diabetes Mellitus, intestinal tract infection, diarrhea, Wound Healing, ear infections, fever and throat infections.

Keywords: Antimicrobial activity, Agar well diffusion method, Piper Cubeba Leaves, Human pathogen.

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