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Abstract

REVIEW ARTICLE: METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMPICILLIN AND CLOXACILLIN CAPSULES BY USING RP-HPLC

M. Sandya Madhuri*, Kuppili Sri Kavya, K.S.M.L.S Kavya and Korukonda Rama Priyanka

ABSTRACT

A simple, accurate, precise, and rapid reverse phase high performance liquid chromatographic isocratic method has been developed for the quantification of method AMPICILLIN and CLOXACILLIN capsule (oral dosage form). The chromatographic separation was attained on P. Hypersob C18 (250mmx4.6, 5μm,) with precolumn as the stationary phase at ambient temperature. The mobile phase was composed of acetonitrile and 0.002 M phosphate buffer pH 3.0, 36:64 with a flow rate of 1.2ml/min, and UV detection at 225nm the run time was 10 minutes. The injection volume was 20. The retention time of ampicillin and cloxacillin were 1.32 minutes and 6.1 minutes respectively. The linearity in the ampicillin drug concentration range of 0.1814 μg/mL to 0.2236 μg/mL and cloxacillin the drug concentration range is 0.1718 μg/mL to 0.2584 μg/mL. The correlation coeffiecient were greater than 0.99. The mean percentage recovery of ampicillin and cloxacillin at 80%, 100%, and 120%concentration levels were for 105.43%, 103.78%, 101.36 & 105.27 and 108.69% respectively. The results obtained show a method that is simple, rapid, and precise. It can be used for the estimation of Ampicillin, Cloxacillin in single as well as combination dosage forms.

Keywords: Ampicillin, Cloxacillin, RP-HPLC, Validation.


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