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Abstract

METHOD DEVELOPMENT AND VALIDATION OF DICLOFENAC SODIUM BY USING UV SPECTROSCOPY

Dr. Kuna Mangamma*, Sontena Sreelekha, Kuppili Sri Kavya, Kalidindi Sita Maha Lakshmi S. Kavya and Pampana Lalitha Bharathi

ABSTRACT

A simple, selective, accurate, precise spectroscopic method for the estimation of diclofenac sodium in bulk and pharmaceutical tablet dosage form has been developed and validated. The linearity range of diclofenac is 10-30μg/ml. The LOD and LOQ were found to be 1.03g/ml and 3.12μg/ml respectively. The amount of diclofenac was calculated as 99.42%. Further the precision of the method was confirmed by the repeatable analysis of solution. The % RSD was found to be 1.370 it indicated that the method has good precision. The % recovery was found to be in the range of 98.72-99.15%. the % recovery was calculated for 80%, 100% and 120% RSD value indicated that there is no interference due to excipients used in formulation. Hence the accuracy of the method was confirmed.

Keywords: Diclofenac sodium, UV method, validation.


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