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Abstract

REVIEW ON HYPHENATED TECHNIQUE

Pallavi S. Thombare*, Ramanlal N. Kachave and M. J. Chavan

ABSTRACT

Hyphenated separation techniques refer to a mixture of two or more methods for separating and detecting chemicals from solution. And biochemistry hyphenated methods are commonly implemented. In 1980, Hirsh Feld first adopted the term “hyphenation” to describe a probable combination of two or more instrumental research methodologies in a single run (Hirshfeld, 1980). The purpose of the pairing is to gain an information rich detection with a single analytical technique for both identification and quantification. To obtain strength of both, hyphenated technique combines chromatographic and spectral processes. In a mixture, chromatography produces pure or almost pure fractions of chemical components. Spectroscopy uses standards or library spectrums to create selective information for analysis.

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