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Susrita Sharma*, B. Ray and C. K. Sahoo


Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. There are two methods for obtaining cells: from a cell bank or by isolating cells from donor tissue. When starting culture from cells obtained from a cell bank, one needs to go through the procedures of "thawing," "cell seeding" and "cell observation." culture techniques is vital tool in the process of drug Discovery which ultimately leads to quantify the steps of analysis of therapeutic potential of drugs. Gene therapy depends on the analysis of cell culture to disclose the unidentified facts related to genomics. The present review Focus different types of cell culture and related technique. It also highlights Recent progress in the particular field.

Keywords: Cell cultue, Immunolabeling, genomics, cell seeding, Biological buffer.

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