Abstract

EVALUATION OF PHYTOCHEMICALS, ANTIOXIDANT POTENTIAL AND INVITRO ANTIBACTERIAL EFFICACY OF MURRAYA KOENIGII LEAVES AGAINST UROPATHOGENIC E.COLI

M. Veera Pagu*, K. R. Jeya and S. Vennila

ABSTRACT

Urinary tract infection (UTI) is the second most common infectious disease prevailing in developed and developing countries. Although a number of antimicrobial agents are available for the treatment and management of urinary tract infection, some microbes develop resistance to many antibiotics. Murraya koenigii (Curry leaves) a commonly used medicinally important herb of Indian origin in the Ayurvedic system of medicine are rich sources of carbazole alkaloids, which produce potent biological activities and pharmacological effects. Hence the present study were undertaken to investigate phytochemical screening, antioxidant activity and antimicrobial acitivity of aqueous extracts of Murraya koenigii leaves against the uropathogenic E.coli isolate from the urine samples of urinary tract infected patients. The results showed the presence of alkaloid, carbohydrate, flavonoids, phenols, phylobatannins, tannins, aminoacids and proteins in phytochemical analysis, exhibited four peaks 751.55, 954.65, 1,008.20 and 1,053.00 in UV-Vis analysis and characteristic peak in FTIR determination revealed the presence of alcohols, phenols, alkene, alkane, aromatics, amines etc. The minimum inhibitory concentration (MIC) of crude curry leaf aqueous extract was found to be10mg/ml against uropathogenic E.coli, MBC 20mg/ml, MBC/MIC value 2 and total antioxidant activity (TAC) 27.29 AAE/100 g. Thereupon Murraya koenigii can be exploited as a potent therapeutic agent against uropathogenic E.coli.

Keywords: Urinary tract Infection (UTI), Murraya koenigii, Curry leaves, Uropathogenic E.coli, MIC, MBC.