PHYTOCHEMICAL EVALUATION AND HPTLC FINGER PRINTING PROFILE OF LEAVES OF FILICIUM DECIPIENS
Kavitha S.* and Parvathavardhini D.
ABSTRACT
A plant derivative plays a vital role in the modern pharmaceuticals for
various ailments. High performance thin layer chromatography
(HPTLC) is a valuable technique for the investigation of plant products
with respect to different aspects of their quality. The present study was
carried out to investigate the secondary metabolites and to develop
HPTLC. To study the HPTLC fingerprinting of leaf extract of Filicium
decipiens, A CAMAG HPTLC system equipped with LINOMAT 5
applicator and WIN CATS-1.3.4 software were used. The
Phytochemical tests was done on methanol, ethanol and chloroform
extracts. Preliminary Phytochemical studies shows the presence of
various phytoconstituents like alkaloids, saponins, flavonoids, phenols
and tannins and the three solvent extract were used for the further
analysis. HPTLC study revealed the presence of more bands in
chloroform than methanol and ethanol extract of Filicium decipiens. The HPTLC
fingerprinting profile developed for chloroform extract will help in proper identification and
quantification of marker compounds. By isolating and identifying marker compounds, it can
be used for the formulation of new drugs to treat various diseases.
Keywords: Filicium decipiens, HPTLC, fingerprinting, phytochemical screening.
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