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Abstract

DETECTION OF DENGUE VIRUS FROM F1 GENERATION OF FIELD COLLECTED LARVAE FROM DWARKA AND ROHINI, DELHIā€ AT NATIONAL INSTITUTE OF MALARIA RESEARCH (ICMR).

*Nilu Kumari, Dr. Himmat Singh, Dr. Amit Kumar Pandey, Dr. Sneha Pandey

ABSTRACT

Dengue is one of the most dangerous infectious disease and it
emerging as the major public health concern in various parts of India.
The aim of our study was to detect dengue virus in F1 generation of
field collected larvae from Dwarka and Rohini, New Delhi. We
collected larvae from different inside and outside placed containers on
the basis of their characteristics and selection of habitat from different
localities of Dwarka and Rohini. 10 or less than 10 mosquitoes were
used as a pool. Then, these mosquitoes undergo the RNA-Extraction
method by using TRIZOL. The collected RNA samples were then
tested for DENV presence using RT-PCR Techniques. RT-PCR is the
modification of PCR techniques and is used to amplify a single
stranded RNA template to yield abundant double stranded complimentary DNA products.
After RT-PCR, the the PCR product is separated through AGAROSE GEL
ELECTROPHORESIS and the sample runs on the solidified surface of agarose and they
visualize under the GEL DOCUMENTATION on the basis of their band formation at Base
PAIR ABOVE 500, detected as the positive sample, confirms the presence of dengue virus in
the mosquito of BAGHDOLA, Dwarka, Delhi represented positive result of our study.

Keywords: Dengue, AGAROSE GEL ELECTROPHORESIS, Aedes aegypti, Aedes albopictus.


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