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Abstract

A COMPREHENSIVE REVIEW ON GENE TRANSFER TECHNIQUES: AN OVERVIEW

Imad Ahmad*, Amresh Gupta, Amit Kishor Srivastava, O. P. Verma and Neelam Verma

ABSTRACT

Biolistic are developed as a system for gene delivery into plant cells, but has recently been introduced for transfection in to mammalian tissue, including few attempts in neural cells. Basically, in this system the plasmid DNA of interest is coated on to small particles, that are accelerated by a particular driving force. The combination of several so-called ‘ballistic’ parameters and tissue parameters determines the transfection efficiency. In terms of transfection efficiency, biolistic seems favorable above conventional techniques, like calcium phosphate precipitation and lipofection. Compared to viral techniques biolistic may be less efficient, but is quicker and easier to handle and seems to produce fewer complications for in vivo gene delivery. Therefore, although the technique is only in a development al stage. Preliminary results seem promising, and optimization of the method may prove useful in scientific research and/or clinical use.

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