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Abstract

COMPARATIVE STUDY ON THE ANTI-SHIGELLOSIS ACTIVITY OF BAY (Syzygium polyanthum [Wight.] Walp.) and SOYBEAN (Glycine max (L.) Merrill) LEAVES EXTRACT

Sri Agung Fitri Kusuma*, Danni Ramdhani, Rudi Satria and Putu Listynelia Wirda Andanseswari

ABSTRACT

Objective: This aim of this study was to compare the anti-shigellosis of bay (Syzygium polyanthum [Wight.] Walp.) and soybean (Glycine max (L.) Merrill) leaves extract against Shigella dysenteriae ATCC 13313. Methods: Each of the dried bay and soybean leaves were extracted using 70% ethanol by a maceration method. Phytochemical content of the extracts was analyzed by the standard phytochemical assays. Determination profile the thin-layer chromatography (TLC) was performed using different solvent system. The extracts were then tested for the anti-shigellosis activity against S. dysenteriae ATCC 13313 using the agar diffusion method. The potential inhibition of both tested extracts was compared. Results: The different phytochemical substances were detected in both leaf extracts. The bay leaf extracts contained flavonoid and tannins, meanwhile flavonoid, polyphenols, monoterpenoid, and sesquiterpenoid were detected in the soybean leaf extracts. However, the antibacterial test results showed that the ethanol extract of bay leaves produced higher diameter of inhibition than soybean leaf extract. Conclusion: The leaves extract of bay is a potential natural agent to be an antishigellosis candidate compared to soybean leaf extract.

Keywords: comparative, Syzygium polyanthum [Wight.] Walp, Glycine max (L.) Merrill, Shigella dysenteriae.


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