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A. Prudhvi Sai Krishna*, Y. Srinivasa Rao, K. Varaprasada Rao, R. Deepthi and D. Vasudha


Affinity Chromatography is a type of separation technique which is widely used to analyze the pharmaceutical substances and biological substances. The main principle involved here is based upon affinity. The separation is based on highly specific macromolecular binding interaction between the biomolecule and substance. The interaction is based upon the analyte of interest. The biomolecule interaction takes place between antigen and antibody, enzyme and substrate, ligand and receptor. This chromatographic method is used to purify the molecules in a complex mixture by specific interactions with the ligand. It is used in the separation of pharmaceutical, therapeutically, clinical and biological samples. Affinity chromatography has advantage for its high specificity and selectivity and resolution of separation compared to other chromatographic methods.

Keywords: Affinity chromatography, matrix, ligand, spacer arm.

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