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Abstract

INSTRUMENTATION AND FUTURE PROSPECTS OF HPTLC- A REVIEW

Pallavi Ambati*, Bala Krishna P., Srinivasa Rao Y., Varaprasada Rao K. and Deepthi R.

ABSTRACT

High Performance Thin Layer Chromatography (HPTLC) technique is a sophisticated and automated form of thin-layer chromatography (TLC) with better and advanced separation efficiency and the detection limits and it is an excellent alternative to GC and HPLC. Application of HPTLC includes the phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. HPTLC useful in detecting substances of forensic concern. Various advance techniques in reference to HPTLC like hyphenations in HPTLC-MS, HPTLC-FTIR and HPTLC-Scanning Diode Laser have made HPTLC a powerful analytical tool in the field of analysis. Experts are the opinion that HPTLC future to combinatorial approach and the utilization of instrumental HPTLC toward the analysis of drug formulations, bulk drugs, and natural products will increase in the future.

Keywords: High Performance Thin Layer Chromatography (HPTLC), Analytical tool, Herbal, Forensic, Hyphenation.


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