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Nagendra Chary M.*, Lalitha B. R. and T. Anil kumar


Background: In Ayurveda, medicated ghee is one the dosage form which focuses on the male infertility issues. Madhyama panchamoola is a group of five drugs consist of Bala (Sida cordifolia) Punarnava (Boerhavia diffusa), Eranda (Ricinus communis) Mudgaparni (Vigna trilobata) and Mashaparni (Teramnus labialis). Each of these drugs possess Rasayana (Rejuvenation), Vrushya, (Aphrodisiac) and Shukrajanana (Spermatogenic) activities. Madhyama panchamoola sadhita ghrita helps in treating Oligospermia. Gas chromatography – Mass Spectrometry is the proven analytical method which is highly effective and versatile to identify different components in a ghee based samples. Objective: The present study deals with the Gas
Chromatography Mass Spectrometry (GC–MS) analysis of Madhyama panchamoola sadhita ghrita. Materials and Methods: The Madhyamapanchamoola sadhita ghrita was prepared by following Ayurveda formulary of India with GMP certified company and subjected to GC–MS analysis. Results: GC-MS revealed total 19 compounds. Glycerol, Squalene, Lanosterol (Lupeol) were experimentally proved to have Antioxidant and Spermatogenic activities. Conclusion: The Phytochemical constituents identified in the GC–MS profile revealed Madhyama panchamoola sadhita ghrita has a potential Shukra janana activity.

Keywords: Madhyama panchamoola sadhita ghrita, GC-MS, Spermatogenic activity.

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