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Abstract

ANTICANCER EFFECT OF SAPONIN RICH FRACTION FROM HELIANTHUS ANNUUS: AN IN SILICO AND IN-VITRO STUDIES

Desai Tanvi H.* and Joshi Shrikant V.

ABSTRACT

Objectives Presented study was performed to explore the anticancer activity of saponin rich fraction of Helianthus annuus flower petals using in silico studies, MTT assay, shell-less chick embryo culture assay, chromosomal aberration assay and apoptosis assay. Methods In silico studies of helianthosides (A-C) were performed for the prediction of structure based pharmacological activities using PASS Online and Swiss Target Prediction softwares for the possibilities to be anticancer and apoptogenic along with docking studies using Autodock Vina. MTT assay was executing for antiproliferative action of saponin rich fraction on MCF-7 cells. For the evaluation of antiangiogenic properties, in vitro shellless chick embryo culture assay was studied. chromosomal aberration was studied on normal human blood. Apoptosis assay was performed on MCF-7 cells using cell based ELISHA assay kit. Result MTT assay displayed IC50 value in MCF-7 cells at 2μg/mL. Considerable (p<0.05) decreases in angiogenic parameters were observed after treatment. Non significant aberrations were noticed following the treatment. Treatment causes activation of caspase 3 and caspase 8 (OD 0.42 and 0.33 respectively at 450 nm) in MCF-7 cells. Conclusion Saponin rich fraction of H. annuus showed antiproliferative, antiangiogenic and apoptogenic potential in in vitro models with non significant chromosomal aberrations in normal cells.

Keywords: Helianthus annuus, MTT assay, Antiangiogenic assay, Chromosomal aberration assay, Apoptosis assay.


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