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Shariful Haque*, Monira Ahsan and Choudhury Mahamud Hasan


Two compounds 4, 9 –dihydroxy aromadendrene and 4-α-hydroxy aromadendrene were isolated from the n-hexane soluble partitionate of methanol extract of rhizome of Cyperus articulatus. At first the powdered rhizome of C. articulatus was extracted with 95% methanol. An aliquot of the extract was chromatographed over silica gel (Kiesel gel 60H) and vacuum liquid chromatography (VLC) column was eluted with n-hexane and ethyl acetate mixtures of increasing polarities to give a total of 18 fractions, each 100 ml. VLC fraction 6 was subjected to preparative thin layer chromatography (PTLC) using toluene-ethyl acetate (80:20) as solvent system. From the developed plates the bands were scrapped off and eluted with same solvent system- Toluene-Ethyl acetate (80:20), a 50:50 mixture of ethyl acetate and chloroform followed by 100 % ethyl acetate. The structures of the compounds were elucidated by high field NMR studies as well as comparison with spectral data of related compounds.

Keywords: Cyperus articulatus, aromadendrene, Cyperaceae, VLC, PTLC, NMR.

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