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Wathiq Abass Al-Daraghi, Lina Mohammed Jaffer* and Wisam H.Salo


This study used 200 Different samples including, 100 soil samples collected from Basrah and Ramadi governorate in to areas already exposed to radiation as a result of wars; 100 swab samples, which were collected from two hospitals in Baghdad (Kamal al-Samarrai and Radiation and Nuclear Medicine Hospital) from the operating rooms, equipment and medical instruments after ultraviolet exposure as a means of fogging. Molecular diagnosis was conducted by isolation of DNA content and detection of 16 rRNA gene by using a Deinococcus specific hemi-nested primer, then detection of RecA gene using polymerase chain reaction (PCR). The results revealed (100% isolates) from both Bsraha and Ramadi soil samples. Results agree with the routine assays carried out the Deinococcus Radiodurans isolate from Kamal al-Samarrai Hospital and Radiation and Nuclear Medicine Hospital) showed that all of them carry multiple different isolates but they included Deinococcus Radiodurans isolate between (30-10 %) from the 100 swab samples. One of the aims of this study was to adopt an accurate diagnostic method to detect Deinococcus Radiodurans by its genetic material contents through extracting DNA and gel electrophoresis of the PCR product for the specific gene. Detection of radiation-resistance gene represented by a confirmatory test was carried out for the selected isolates using the Hemi-nested polymerase chain reaction technique for further characterization up to the species level by the amplification of (Eub27F and Eub1107R) genes .These are specific genes that encode the universal eubacterial characteristic which is unique to DNA damage agents. The result of (Eub27F and Eub1107R) genes were used as a template for the Deinococcus Radiodurans specific primer (Deino202F) gene then detection of (RecA) gene. All the positive isolates by the routine tests were found to be positive for the molecular tests: Presence of (Eub27F and Eub1107R) genes as their agarose gel revealed with a molecular size about (1100 bp), (Deino202F) gene with a molecular size about (900bp) and (RecA) gene with molecular size about (254bp).

Keywords: Deinococcus Radiodurans specific hemi-nested primer, Eub27F and Eub1107R.

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