EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF PERGULARIA DAEMIA
Sridevi G, Sembulingam K, Muhammed Ibrahim, Srividya S, Prema Sembulingam*
ABSTRACT
Pergularia Daemai (PD) is an age-old traditional herb and is in use as
folk medicine among the villagers even today. It had been screened for
its bioactive compounds and various pharmacological activities. But its
anti-inflammatory activity is not explored fully. In the present venture,
we evaluated the anti-inflammatory effect of ethanolic extract of PD
by in vitro method by using membrane stabilization test and protein
denaturation test. Membrane stabilization test was done by using
human red blood cells (HRBCs). Protein denaturation test was done by
using bovine serum albumin (BSA). The results revealed that PD
extract was capable of rendering membrane stabilization by inhibiting
the hypotonically-induced hemolysis of HRBCs in dose-dependent
manner (50, 100, 200, 300, 400, 500 and 1000 μg/mL). In lesser
concentration (50 μg/mL), the % inhibition of hemolysis was less
(26.80%) and in higher concentration (1000 μg/mL), the % inhibition
of hemolysis was more (76.30%), which was comparable with that of standard antiinflammatory
drug viz. diclofenac sodium (200 μg/mL – 80.60%). The PD extract was also
capable of inhibiting BSA denaturation in dose-dependent manner (50 μg/mL – 20.40%,
1000 μg/mL – 83.60%) which was comparable to that of diclofenac sodium (200 μg/mL –
86.60%). This finding confirms the potentiality of PD extract as an anti-inflammatory agent and justifies the recommendation of PD extract for the treatment of painful inflammatory
conditions.
Keywords: Anti-inflammatory, Pergularia daemia, In vitro, Diclofenac sodium.
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