Abstract

EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY OF PERGULARIA DAEMIA

Sridevi G, Sembulingam K, Muhammed Ibrahim, Srividya S, Prema Sembulingam*

ABSTRACT

Pergularia Daemai (PD) is an age-old traditional herb and is in use as folk medicine among the villagers even today. It had been screened for its bioactive compounds and various pharmacological activities. But its anti-inflammatory activity is not explored fully. In the present venture, we evaluated the anti-inflammatory effect of ethanolic extract of PD by in vitro method by using membrane stabilization test and protein denaturation test. Membrane stabilization test was done by using human red blood cells (HRBCs). Protein denaturation test was done by using bovine serum albumin (BSA). The results revealed that PD extract was capable of rendering membrane stabilization by inhibiting the hypotonically-induced hemolysis of HRBCs in dose-dependent manner (50, 100, 200, 300, 400, 500 and 1000 μg/mL). In lesser concentration (50 μg/mL), the % inhibition of hemolysis was less (26.80%) and in higher concentration (1000 μg/mL), the % inhibition of hemolysis was more (76.30%), which was comparable with that of standard antiinflammatory drug viz. diclofenac sodium (200 μg/mL – 80.60%). The PD extract was also capable of inhibiting BSA denaturation in dose-dependent manner (50 μg/mL – 20.40%, 1000 μg/mL – 83.60%) which was comparable to that of diclofenac sodium (200 μg/mL – 86.60%). This finding confirms the potentiality of PD extract as an anti-inflammatory agent and justifies the recommendation of PD extract for the treatment of painful inflammatory conditions.

Keywords: Anti-inflammatory, Pergularia daemia, In vitro, Diclofenac sodium.