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Lubaina AS, Soumya R. Raj, Brijithlal ND and Murugan K*


Bryophytes include mosses, liverworts and hornworts and are the second largest group of plants, with approximately 28.000 species worldwide. Bryophyte chemistry is poorly documented, and are scattered. They are considered as remarkable reservoir of new, secondary compounds, many of which have shown interesting biological activities. Therefore, the present investigation was undertaken to analyse the various phytochemicals qualitatively and quantitatively using various solvents such as water, ethyl acetate and petroleum ether of the moss Pogonatum microstomum and its bactericidal activities. Initially the different extracts revealed a pool of phytochemicals. Quantification data revealed significant levels of phenols and flavonoids. In the second phase, the total phenols were fractionated by RP- HPLC analysis, which revealed the presence of phenolic acids such as sinapic acid, hydroxycinnamic acid, vanillate, gallate, chlorogenate, ferullate, phlorogucinol and catechol. Varying levels of bactericidal potentialities was displayed by the three different extracts against Streptococcus haemolyticus, Staphylococcus aureus, Klebsiella pneumoniae, and Escherichia coli using disc diffusion method and the results were comparable with synthetic antibiotic. Further studies are warranted to elucidate, purify the lead molecule and to explore their bactericidal mechanism.

Keywords: Phytochemicals, bactericidal activity, RP- HPLC analysis, Klebsiella pneumoniae, Streptococcus haemolyticus.

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