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Sadiq R. M. Ali*, Ahmed J. Fradi and Alaa M. Al-Aaraji


The present study was aimed to Determinated the best cultural medium. In this paper, Aspergillus was choice to conduct this study as it is a widespread and important and conducted by many studies, and chosen five cultural media because it the most widely used in various research and cultivation of fungi used for different purposes. The growth rate of five Aspergillus species, grown on five different culture media were observed after 7 days of incubation at 29±1°C. The colony diameter of fungi were greatly influenced by the type of culture medium used. Five culture media were used to determinated the best media. The results of tested done on five species of Aspergillus, showed that the best media was SDA, PDA, Czapek, MEA and CMA respectively. Sabouraud dextrose agar (SDA), this media was use for routine cultivation and used for growing and saving the fungal isolates. Potato dextrose agar (PDA), this media was used for routine cultivation and identification of fungi. Czapek’s dox agar, is used for the cultivation of fungi. Malt extract agar medium (MEA), a medium for the detection, isolation and enumeration of yeasts and moulds. Corn meal agar medium (CMA), this medium was used for differentiation between certain strains of fungi. The results will be useful for choice the best media and fungal taxonomic studies.

Keywords: Aspergillus, culture media.

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