Abstract

ANTIPLASMODIAL AND DPPH RADICAL SCAVENGING EFFECTS IN EXTRACTS FROM ACACIA MACROSTACHYA (MIMOSACEAE) DC

Tondé Issa, Fofana Souleymane*, Gnoula Charlemagne, Palé Eloi, Nébié Roger HC, Nikiema Jean Baptiste, Simpore Jacques and Guissou Innocent Pierre

ABSTRACT

Background: Acacia macrostachya is a medicinal plant used in the traditional treatment of snakebites and pathologies accompanied with oxidative stress such as malaria, inflammation, and painful spasms. Objective: The purpose is to study the antioxidant and antiplasmodial effects in extracts of leaves and bark of twigs from Acacia macrostachya. Methodology: The extracts were prepared by exhaustion using dichloromethane, dichloromethane / methanol (1:1), methanol, methanol / water (1:1) and water. The antioxidant effects were assessed by the 1, 1 diphenyl-2-diphenyl picrylhydrazyl (DPPH) test. In order to prove the antiplasmodial effect in extracts using the optical microtest, wild clinical strains of Plasmodium falciparum have grown according to the method of Trager and Jensen. Sterols / triterpenes, flavonoids, tannins, alkaloids, and saponins were subsequently sought for. Results: All leaf extracts were strongly antioxidant often comparable to quercetin (IC50-Quercetin: 2. 63μg/mL versus IC50- MeOH-leave: 1. 11μg/mL). Extracts from bark in dichloromethane (IC50: 14. 92μg/mL) and methanol (IC50: 11. 40μg/mL) were also active. The best antiplasmodial activities were found in the hydro-methanolic extracts of leaves (IC50: 5. 06μg/mL) and bark (IC50: 4. 92μg/mL). Extracts of A. machrostachya have proved very rich in saponins, rich in flavonoids, tannins and alkaloids A. machrostachya, antioxidant, antiplasmodial, Burkina Faso.

Keywords: A. machrostachya, antioxidant, antiplasmodial, Burkina Faso.