SODIUM SELENITE ATTENUATES NICOTINE-INDUCED OXIDATIVE STRESS IN RAT
Sankar Kumar Dey*, Durga Pada Dolai, Debasis Mandal, Balaram Das, Sandeep Kumar Dash and Somenath Roy
ABSTRACT
Nicotine, a major toxic component of cigarette smoke has been
identified as a major risk factor for different diseases. In the present
study, we evaluated the protective effects of sodium selenite on lipid
peroxidation and antioxidants status against nicotine treated liver and
kidney toxicity. A group of male Wistar rats were induced by
subcutaneous injection of vehicle, nicotine (2mg/kg body weight/day)
and nicotine plus sodium selenite (0.5 mg/kg of diet) for the period of
15 days. Measurement of biochemical marker enzyme like lactate
dehydrogenase (LDH), lipid peroxidation (MDA), conjugated dienes
(CD) and antioxidants were used to monitor the antiperoxidative effect
of sodium selenite. The decreased biochemical marker enzyme as well
as increased lipid peroxides and conjugated dienes in liver and kidney
of nicotine treated rats was accompanied by a significant decrease in the levels of glutathione
(GSH and GSSG) and the activities of glutathione peroxidase (GPx), glutathione reductase
(GR), glutathione-s-transferase (GST), superoxide dismutase (SOD) and catalase (CAT).
Dietary co-administration of sodium selenite significantly supplements the biochemical
marker enzyme, lipid peroxidation and enhanced the antioxidant status. The results of the
present study suggest that sodium selenite exert its protective effect against nicotine-induced
liver and kidney toxicity by modulating the biochemical marker enzyme, lipid peroxidation
and augmenting antioxidant defense system.
Keywords: Nicotine, Sodium selenite, Tissues, Oxidative stress.
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