Abstract

PHYTOCHEMICAL CHARACTERIZATION AND INVITRO ANTIOXIDANT EFFICACY OF SARGASSUM WIGHTII

R. Rajeswari* and K. Jeyaprakash

ABSTRACT

Marine seaweeds contain many active components that are identified by the phytochemical analysis. Sea weeds of genus Sargassum has been widely used as a resource for food and medicine. In medicine it heals to cure many diseases. Sargassum Wightii was used for the study of phytochemical analysis. The extractions of phytochemicals from Sargassum wightii were carried out with different solvents such as Ethanol, Methanol and Water. The preliminary screening revealed the presence of tannin, saponin, flavonoids, steroids, terpenoids triterpenoids, alkaloids, carbohydrate, protein, polyphenol and glycosides with varied degree. It was found that the aqueous extract of shows better results than the other. The total phenol and flavanoid determination were 271 and 209 mg/g. The invitro antioxidant activity of Sargassum wightii was measured by DPPH radical scavenging assay, superoxide anion scavenging assay, Hydroxyl radical scavenging assay, Nitric oxide scavenging assay, reducing power assay. The results of the present study confirmed that Sargassum wightii may be the rich sources of phytoconstituents which can be isolated and further screened for various biological activities.

Keywords: Phytochemicals, Antioxidant, Sargassum wightii.