Abstract

COMPARATIVE ANTIFUNGAL ACTIVITIES OF SPECIFIED AND MODIFIED SECONDARY METABOLITES OF PAEONIA SUFFRUTICOSA ROOT EXTRACT AGAINST ASPERGILLIUS NIGER UNDER DIFFERENT CONDITIONS

F. Rehman*, Samya Mairaj and U. K. Jetley

ABSTRACT

Paeony suffraticosa is a well known medicinal plant the worldover. Pharmaceutical importance of paeonia suffraticosa is due to the presence of several valuable secondary metabolites in it. Paeonol is one of the active ingredients of the root homogenate of the plant, showing a wide spectrum activity. The anifungal activity of different concentrations of the alcoholic extract of the specified secondary metabolites and their oxime-form against Aspergillus niger fungal strains was studied at different conditions of temperature and pH. The (bio) activity index (A.I) was also derived, and IC50 obtained for 0.04 to 0.004 % concentrations of alcoholic extract of the phenolic compounds and their modified form. The minimum inhibitory concentration (MIC) was also calculated which showed variation with experimental conditions. The results indicate that the fungal strain has different tolerance to solvent, temperature and pH and the above said compounds have good antifungal properties as compared to a standard drug (Flucanazole). The susceptible metabolic pathway was assessed by the determining the structural components glucosamine and ergosterol. The antifungal efficacy could be maintained for longer time only at low temperature.

Keywords: Paeonia Suffraticose, antimicrobial activity, Richard’s liquid medium.