COMPARATIVE ANTIFUNGAL ACTIVITIES OF SPECIFIED AND MODIFIED SECONDARY METABOLITES OF PAEONIA SUFFRUTICOSA ROOT EXTRACT AGAINST ASPERGILLIUS NIGER UNDER DIFFERENT CONDITIONS
F. Rehman*, Samya Mairaj and U. K. Jetley
ABSTRACT
Paeony suffraticosa is a well known medicinal plant the worldover.
Pharmaceutical importance of paeonia suffraticosa is due to the
presence of several valuable secondary metabolites in it. Paeonol is
one of the active ingredients of the root homogenate of the plant,
showing a wide spectrum activity. The anifungal activity of different
concentrations of the alcoholic extract of the specified secondary
metabolites and their oxime-form against Aspergillus niger fungal
strains was studied at different conditions of temperature and pH. The
(bio) activity index (A.I) was also derived, and IC50 obtained for 0.04
to 0.004 % concentrations of alcoholic extract of the phenolic
compounds and their modified form. The minimum inhibitory concentration (MIC) was also
calculated which showed variation with experimental conditions. The results indicate that the
fungal strain has different tolerance to solvent, temperature and pH and the above said
compounds have good antifungal properties as compared to a standard drug (Flucanazole).
The susceptible metabolic pathway was assessed by the determining the structural
components glucosamine and ergosterol. The antifungal efficacy could be maintained for
longer time only at low temperature.
Keywords: Paeonia Suffraticose, antimicrobial activity, Richard’s liquid medium.
[Full Text Article]