ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF CITRUS LIMON L. PEELS
Kutaiba Ibrahim Alzand and Mansor Mostafa Boufaris*
ABSTRACT
To evaluate the antimicrobial activity of the peels of Citrus limon Linn
(CL) against some microorganisms - bacteria and fungus were
Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia,
Pseudomonas spp, Aspergillus niger, Aspergillus fumigates, Mucor
spp and Pencillium. 100 μL of 10 mg CL were assessed against eight
test microorganisms by agar well Diffusion Method. Gentamicin and
Ketoconazole 10 mg/mL were used as standards. A different solvent
was used to obtain CL peel extract by using maceration technique.
%yield obtained for dried peel extract of CL with chloroform, ethanol,
acetone, petroleum ether and aqueous ethanol was approximately 15%,
18%, 09%, 11% and 24% respectively. Due to its high yield value
hydroalcoholic extract of CL was used for estimating the antimicrobial activity and its
phytochemical screening. Phytochemical screening of CL plant reveals the presence of
alkaloids, flavonoids, steroids and tannins. The study demonstrates that the hydroalcoholic
extract of CL peel exhibit antibacterial activity on Klebsiella pneumonia, Pseudomonas sp,
Staphylococcus aureus and antifungal activity among Aspergillus niger, Aspergillus
fumigates, Mucor species. These recognized a good support to the use of this plant in herbal
medicine and as base for the development of new drugs and phytomedicine.
Keywords: Antimicrobial activity, Citrus lemon peels, agar well diffusion method, phytochemical screening, herbal medicine, phytomedicine.
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