Abstract

DETERMINATION OF ELETRIPTAN IN RABBIT PLASMA BY LCMS/ MS AND ITS APPLICATION TO PHARMACOKINETIC STUDY

Praveen Kumar Dasari, Usha Kiranmayi Mangamuri, Vijayalakshmi Muvva, Sudhakar Podha*

ABSTRACT

The proposed method is selective, simple, sensitive and specific Liquid chromatography–tandem mass spectrometry method for the quantification of Eletriptan (EP) in rabbit plasma using Naratriptan (NP) as internal standard (IS). Chromatographic separation was performed on Luna®, C18, 50 × 4.6mm, 2.7μm, Phenomenex column and mobile phase composed of 10mM ammonium acetate, (pH-6.5) and acetonitrile (35:65, v/v), with 0.8 mL/min flow-rate. Drug and IS were extracted by protein precipitation extraction. EP and NP were detected with proton adducts at m/z 383.284.3 and 336.297.8 in multiple reaction monitoring (MRM) positive mode respectively. The method was validated over a linear concentration range of 0.05-210ng/mL with correlation coefficient (r2) ≥ 0.998. This method demonstrated intra and inter-day precision within 2.12 to 7.07% and 1.50 to 5.73% and % Accuracy within 99.29 to 102.00 and 100.00 to 102.21% for EP. The drug was found to be stable under various processing and storage conditions. The developed method was employed in preclinical pharmacokinetic evaluation of eletriptan in rabbit plasma.

Keywords: Eletriptan; rabbit plasma; LC-MS/MS.