DETERMINATION OF ELETRIPTAN IN RABBIT PLASMA BY LCMS/ MS AND ITS APPLICATION TO PHARMACOKINETIC STUDY
Praveen Kumar Dasari, Usha Kiranmayi Mangamuri, Vijayalakshmi Muvva, Sudhakar Podha*
ABSTRACT
The proposed method is selective, simple, sensitive and specific
Liquid chromatography–tandem mass spectrometry method for the
quantification of Eletriptan (EP) in rabbit plasma using Naratriptan
(NP) as internal standard (IS). Chromatographic separation was
performed on Luna®, C18, 50 × 4.6mm, 2.7μm, Phenomenex column
and mobile phase composed of 10mM ammonium acetate, (pH-6.5)
and acetonitrile (35:65, v/v), with 0.8 mL/min flow-rate. Drug and IS
were extracted by protein precipitation extraction. EP and NP were
detected with proton adducts at m/z 383.284.3 and 336.297.8 in
multiple reaction monitoring (MRM) positive mode respectively.
The method was validated over a linear concentration range of 0.05-210ng/mL with
correlation coefficient (r2) ≥ 0.998. This method demonstrated intra and inter-day precision
within 2.12 to 7.07% and 1.50 to 5.73% and % Accuracy within 99.29 to 102.00 and 100.00
to 102.21% for EP. The drug was found to be stable under various processing and storage
conditions. The developed method was employed in preclinical pharmacokinetic evaluation
of eletriptan in rabbit plasma.
Keywords: Eletriptan; rabbit plasma; LC-MS/MS.
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