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Mallapagu E. Rani


We present the validation of a developed anew spectrophotometric method based on a charge transfer complexation reaction of heterocyclic ring containing drug. In this method, the oxidation of Doxazosin drug by a know excess amount of cerium IV sulphate in acid medium, the unreacted cerium IV sulphate was treated with Iron II sulphate, After ‘5’ minutes the resultant Iron III sulphate was treated with (1M) Ammonium thiocyanate, immediately it forms blood red colour of Iron III sulphate thiocyanate drug complex solution. Which was stable for more than 24 hrs. The resultant blood red colour solution was measuring absorbance at 505 nm against reagent blank, Beer’s law was obeyed for 50-250 ug/ml-2 of Doxazosin. Results of analysis were validated statistically and by recovery studies. The procedures described were successfully applied to the determination of Doxazosin for tablets.

Keywords: Doxazosin spectrophotometric cerium IV sulphate, Iron III sulphate (ammonim ferrous sulphate solution) Ammonium thiocyanate solution.

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