PHARMACOGNOSTICAL STUDY AND EVALUATION OF ANTIBACTERIAL ACTIVITY OF SOPHORA INTERRUPTA BEDD
Angilicam Avinash*, Samudrala Sudheer, Ganipineni Sumanth, Gundala Vinod Kumar, R. Ramasubramania Raja, Dr. M. Sreenivasulu and G. Madan Mohan
ABSTRACT
Sophora interrupta Bedd, was a common woody perennial herb native
to India which belongs to Leguminosae family. The present study deals
about anti-bacterial activity of Sophora interrupta. The antibacterial
activity of benzene extract of Sophora interrupta bedd, was carried out
by disc diffusion technique. The root extract was subjected to
preliminary phytochemical screening. The benzene extract of Sophora
interrupta of different concentrations about 0.25, 0.5, 0.75, 1.0,
1.25,1.50mg/ml was made dissolved in DMSO. These solutions were
sterilized using filtration sterilization technique, these dilutions were
used to test the antibacterial activity of 3 different strains viz, Bacillus
subtilis, Pseudomonas aureus and Pseudomonas syringe. The obtained
results were compared with standard drug Ciprofloxacin. The
minimum inhibitory concentration was determined for the concerned microorganism. From
the experimental results it can be concluded that root extract of Sophora interrupta Bedd., has
very good Antibacterial activity.
Keywords: Antibacterial activity, Benzene extract, Ciprofloxacin, Disc diffusion technique, Sophora interrupta.
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