Abstract

CHEMICAL CONSTITUENTS AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF CISTUS LADANIFERUS (L)

Fadila Moussaoui* and Tajelmolk Alaoui

ABSTRACT

The present study aims to assess the in vitro antioxidant activity in the methanol ,water, ethanol extracts and the decoction of leaves of Cistus ladaniferus (L). The antioxidant activity was evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing antioxidant power (FRAP) and chelation power on ferrous ions. The quantitative analysis of total flavonoids and phenols by the colorimetric method shows that polar extracts (methanol and water) and the decoction is the most extracts rich in polyphenols compared with the ethanol extract. The methanolic extract of Cistus ladaniferus has better antiradical capacity (IC50 = (0.047 ± 0.01 mg / ml) in comparison with ascorbic acid (IC50= 0.041 ± 0.07mg / ml). As for the ethanolic extract, it has an ability to reduce iron much lower than that of ascorbic acid. In general, the evaluation of antioxidant indicated that the leaves extracts of Cistus ladaniferus (methanol, water, decoction) showed strong activity to scavenge DPPH radicals, a high reducing power of iron, but very little chelating effect.

Keywords: Cistus ladaniferus; Antioxidant activity; DPPH; FRAP; Chelation power on ferrous ions.