COMPARATIVE STUDY ON IN-VIVO ANTI-INFLAMMATORY POTENTIAL FROM LEAVES OF METHANOL AND CHLOROFORM EXTRACTS OF AZADIRACHTA INDICA A. JUSS AND JUSTICIA ADHATODA IN NEPAL
Shailendra Dhakal*, Pramod Aryal, Sabitri Lamichhane, Dipendra Khadka, Balkrishna Adhikari and Sujan Pandey
ABSTRACT
The present study was carried out to evaluate and compare antiinflammatory
potential in different extract from leaves of plants along
with analysis of phytochemicals responsible for anti-inflammatory
activity. Phytochemical analysis revealed presence of various
phytoconstituents such as- Phenol, flavonoids, glycosides,
carbohydrates, proteins and steroids. Total Phenolic Content (TPC)
was estimated using gallic acid as standard. Total phenolic content was
207.39 ± 8.77 mg GAE/g and 58.08 ± 4.41 mg GAE/g for methanol
and chloroform extract of A. indica respectively while 110.00 ± 6.87
mg GAE/g and 75.80 ± 5.55 mg GAE/g for methanol and chloroform
extract of J. adhatoda respectively. A well established method:
carrageenan induced rat paw edema was adopted for the study of anti-inflammatory activity
where adult Wistar albino rats (110-160)gm of either sex were selected for study. Adult rats
were divided into 10 groups, carrageenan was injected in hind paw and treated with extract at
the standard dose of 200 mg/kg and 400mg/kg. The paw volume was measured at different
time intervals i.e. 0,2,4 and 12 hours. Percentage inhibition of inflammation was proportional
with the total phenolic content. The result indicates that methanol extract of A. indica has
highest anti-inflammatory activity than both extract of J. adhatoda while its chloroform
extract has least activity among all extracts.
Keywords: Phytochemical screening, anti-inflammatory activity, total phenolic content, Azadirachta indica, Justicia adhatoda.
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