METHOD DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF CHLORTHALIDONE HYDROCHLORIDE, AMLODIPINE BESYLATE AND OLMESARTAN MEDOXOMIL IN BULK AND COMBINED TABLETS DOSAGE FORMS
Shweta Hingde* and Dr. Snehalatha Boddu
ABSTRACT
A simple, selective, accurate high Performance Liquid
Chromatographic (HPLC) method was developed and validated for the
analysis of Chlorthalidone HCl, Amlodipine Besylate, and Olmesartan
Medoxomil. The separation was achieved using Prontosil C18 column
(250 x 4.6 mm, 5μm) and mobile phase consisting Buffer and
Acetonitrile in the ratio 40: 60 with pH adjusted to 3.0 with
orthophosphoric acid at flow rate of 1 ml/min. The retention time of
Chlorthalidone HCl, Amlodipine Besylate and Olmesartan Medoxomil
was found to be 3.017 mins, 3.313 mins and 5.010 mins respectively.
The calibration curve were linear over the concentration ranges 26.75-
38 μg/ml, 7.5-20 μg/ml and 30-80 μg/ml for Chlorthalidone HCl, Amlodipine Besylate,
Olmesartan Medoxomil respectively. The proposed method is accurate with 99% recovery
for Chlorthalidone Hydrochloride, 100.20% for Amlodipine Besylate and 98.97% recovery
for Olmesartan Medoxomil in pharmaceutical preparation were all greater than 98% and their
relative standard deviations were not more than 2.0%. Therefore, proposed method can be
used as a more convenient and efficient option for the analysis of all drugs in bulk and tablet
dosage form.
Keywords: Chlorthalidone Hydrochloride, Amlodipine Besylate, Olmesartan Medoxomil, RP-HPLC, Validation.
[Full Text Article]