CHROMATOGRAPHIC SEPARATION OF TANNIN RICH PLANTS AND CHARACTERIZATION OF COMPOUNDS USING ANALYTICAL TECHNIQUES
Rajeswari Anburaj* and Vinoth Jothiprakasam
ABSTRACT
Tannins are polyphenolic compounds found to exhibit antibacterial,
antiviral and antioxidant property. This paper represents an analytical
approach to characterize tannins, phenolic compounds and to assess the
antimicrobial efficacy of the vegetable tannins. Tannins are polyphenol
rich plant substances found to possess defensive roles against
microbes. The results obtained from antimicrobial screening indicates
that K. pneumoniae and C. glabrata remained sensitive towards P.
niruri and T. chebula fraction, whereas C. freundii and F. solani was
found to be resistant. The Uv- vis spectrum indicates the presence of
condensed, gallotannin and ellagic acid. The functional assignments
obtained in FTIR analysis indicates the presence of hydrolysable and
condensed tannin. Hplc chromatogram reveals the presence of
gallotannins such as tetragalloyl glucose, ellagitannin such as pedunculagin and
phenols such as gallic acid and catechin. Therefore phenolic hydroxyl groups located on the
surface of tannin molecules are believed to participate in biological activities of the tannins.
Keywords: Tannin; Antimicrobial assay; phenolic compounds; FTIR; Rp-HPLC.
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