Abstract

CHROMATOGRAPHIC SEPARATION OF TANNIN RICH PLANTS AND CHARACTERIZATION OF COMPOUNDS USING ANALYTICAL TECHNIQUES

Rajeswari Anburaj* and Vinoth Jothiprakasam

ABSTRACT

Tannins are polyphenolic compounds found to exhibit antibacterial, antiviral and antioxidant property. This paper represents an analytical approach to characterize tannins, phenolic compounds and to assess the antimicrobial efficacy of the vegetable tannins. Tannins are polyphenol rich plant substances found to possess defensive roles against microbes. The results obtained from antimicrobial screening indicates that K. pneumoniae and C. glabrata remained sensitive towards P. niruri and T. chebula fraction, whereas C. freundii and F. solani was found to be resistant. The Uv- vis spectrum indicates the presence of condensed, gallotannin and ellagic acid. The functional assignments obtained in FTIR analysis indicates the presence of hydrolysable and condensed tannin. Hplc chromatogram reveals the presence of gallotannins such as tetragalloyl glucose, ellagitannin such as pedunculagin and phenols such as gallic acid and catechin. Therefore phenolic hydroxyl groups located on the surface of tannin molecules are believed to participate in biological activities of the tannins.

Keywords: Tannin; Antimicrobial assay; phenolic compounds; FTIR; Rp-HPLC.