PHYTOCHEMICAL SCREENING, ANTIGLYCATION AND ANTIOXIDANT ACTIVITIES OF ETHANOLIC LEAF EXTRACT OF AZIMA TETRACANTHA LAM.
S. Josephinol*, T. Nargis Begum# and M.H. Muhammad [email protected]
ABSTRACT
Objective: Assessment of in vitro antioxidant and in vitro antidiabetic
potential of Azima tetracantha Lam. Methods: Phytochemicals of
hexane, aqueous and ethanolic extracts of the Azima tetracantha Lam
were analyzed. The antioxidant potential of hexane, aqueous and
Ethanolic extracts was assessed by 2,2-diphenyl-1-picryl hydrazyl
(DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)
(ABTS), ferric reducing ability power (FRAP), superoxide (O2−),
hydrogen peroxide (H2O2)and nitric oxide (NO) scavenging assay and
antidiabetic activity was assessed by α-amylase and α-glucosidase
inhibition. Results: Amongst all the three extracts of A. tetracantha,
ethanol leaf extracts contain significant antioxidant as well as antidiabetic
potential. A. tetracantha showed potential antioxidant as well
as anti-diabetic activities and have good reputation in traditional Indian
medicine. Conclusion: Ethanol leaf extracts of A. tetracanthais having high medicinal
potential in terms of their antioxidant and antidiabetic activities. Studies are required to
further elucidate antioxidant and anti-diabetic potentials using in-vivo biochemical and
molecular biology techniques.
Keywords: Azima tetracantha; DPPH; ?-amylase inhibition; ?-glucosidase inhibition.
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