ANTIOXIDANT, CYTOTOXIC AND PHYTOCHEMICAL ASSESSMENT OF LEAF EXTRACTS OF GOLDEN TRUMPET (ALLAMANDA CATHARTICA L.)
Rashmi Das and Sunita Bhatnagar*
ABSTRACT
Medicinal plants have been used since time immemorial for a number
of diseases. Phytochemical analysis of medicinal plants gives an
insight towards its medicinal potential. In the present study leaf
extracts of one flowering plant Allamanda cathartica was explored for
its phytochemical, cytotoxic and antioxidant activity. On
phytochemical analysis Flavonoids and saponins were present in
chloroform, acetone and methanol extracts. Terpenoids was present in
hexane, chloroform and acetone extract. Tannin was present in fresh,
acetone and methanol extracts. Cardiac glycoside was present in
hexane, acetone and methanol extract. Highest concentration of
phenolic was found in the acetone extract of the plant 124.23μg(GAE)/
g followed by methanol extract with 92.07μg(GAE)/ g. Highest
concentration of flavonoids was found in the acetone extract of the plant 605.50μgQE/g
followed by chloroform extract with 307.16μgQE/g. Cytotoxic activity was significant in
chloroform extract 97.97% at the dose 200μg/ml followed by hexane extract which showed
77.5 % activity at the highest dose. Remaining extracts showed only mild activities against
brine shrimp mortality assay. Highest FRAP value was obtained in methanol extract followed
by acetone extract. However extracts showed mild activity in DPPH radical scavenging
assay. Results indicated in the study points towards a significant medicinal potential of leaf
extracts of Allamanda cathartica.
Keywords: Anti oxidant activity, Phytochemical analysis, Brine shrimp mortality assay.
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