Abstract

ANTIOXIDANT AND HEPATOPROTECTIVE POTENTIAL OF MAMMEA SURIGA AQUEOUS EXTRACT AGAINST CCL4 INDUCED HEPATOTOXICITY IN RAT MODELS

Sudhesh L. Shastri, V. Krishna*, Ravishnakar B., Vinay Kumar N. M. and Chethan Kumara G. P.

ABSTRACT

Mammea suriga is an endemic tree of the family Calophyllaceae, which has important traditional usage as medication. A detailed study was performed on the antioxidant activity of M. suriga flower bud aqueous extract by in vitro chemical analyses and carbon tetrachloride (CCl4) induced hepatotoxicity in Albino rats. The extract was subjected to preliminary phytochemical screening for various constituents which revealed the presence of flavonoids, phenols and terpenoids. The total phenolic contents (79.97 μg/mg) and total flavonoid contents (113 μg/mg) of extract were found to be higher. 200 and 400 mg/kg doses of aqueous extract, CCl4, water and ascorbic acid (100 mg/kg) were used as treatment groups. The blood samples were analyzed for levels of biochemical markers, tissue samples were subjected for estimation of liver antioxidant and histopathological studies. Analysis of the extract treated rats (400 mg/kg) showed an elevation of lipid peroxidation (0.68±0.01 nmol mg-1 of protein). Moreover, the biochemical parameters in serum like SGOT, SGPT, total cholesterol, total creatinine, total protein (TP) and urea were also improved in treated groups compared to the control. The oral administration of doses of M. suriga extract significantly protected the hepatic cells from damage. This study proved that M. suriga could be taken as a good natural source of the antioxidant agent.

Keywords: Mammea suriga, antioxidant activity, hepatotoxicity.