DEVELOPMENT AND VALIDATION OF VISIBLE METHOD FOR ESTIMATION OF AMPICLLIN IN BULK FORMULATION
Chavi Dagar*, G. Rohit Reddy and T. Ramesh
ABSTRACT
A simple, sensitive, accurate, precise and economical visible
Spectrophotometric method was developed and validated for the
estimation of Ampicillin in Bulk form. The method is based on the
reaction of Ampicillin with FC Reagent [Folin Ciocalteu] in the
presence of 10% Sodium Carbonate giving greenish blue colour
chromogen which shows maximum absorbance at 720nm against
reagent blank. The Chromogen obeyed Beer’s law in the concentration
range of 10-50 μg/ml for Ampicillin. The results of the analysis have
been validated statistically and recovery studies.
Keywords: Ampicillin, Folin Ciocalteu (Fc) reagent, Visible Spectrophotometric.
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