Abstract

“PREVALENCE OF BIOFILM FORMATION IN UROPATHOGEN’

Himanshu Trivedi, *Dr. Shweta Sao, Dr. Ramnesh Murthy and Dr. Sagrikapradhan

ABSTRACT

This study was conducted from February to may 2017 to determine the prevalence, risk factors and Biofilm formation of selected bacterial uropathogens among patients with urinary tract infection (UTI) cases in CIMS Hospital, Bilaspur. A hospital-based cross-sectional survey was conducted to assess the risk factors of UTI. In addition, laboratory based work was conducted to determine the prevalence and bacterial uropathogens using standard procedures. The isolates were identified based on their morphological and biochemical characteristics. Urinarytract infection (UTI) is the most commonly acquired bacterial infection. The purpose of this study was to detect biofilm form ation by uropathogenes isolated from UTIs. The isolated bacteria were tested for biofilm production by tube adherence (TA), congoredagar (CRA) methods. Out of 195 urine specimen, 60 urine samples (30.76%) are culture positive for uropathogen with significant colony count. These 60 uropathogen were processed in the present study for biofilm production, 36 (60%) were culture positive and remaining 29 (48.33%) were culture negative. E. coli was the predominant isolate 45/60 (75%) followed by Staphylococcus aureus 2/60 (3.33%), Klebsiella pneumonia 1/60(1.66%), Pseudomonas aeruginosa 2\60(3.33%), Coagulase negative Staphylococci 2\60(3.33%), Enterococcus faecali 4\60(6.66%), Proteus spp 2\60(3.33), Streptococcus spp 1\60(1.66%), Acinetoobacterspp 2\60(2.33%). The antibiotic resistance was higheramong biofilm producers to commonly used antibiotics ascompared to nonbiofilm producers to its detection by congoredagar method. From this study, we have concluded that TM and CRA method is more qualitative and reliable method to detect biofilm producing microorganisms. TM and CRA method is used as a general screening method for detection of biofilm producing bacteria in laboratories. Biofilm form at ion is the major virulence determinant of uropathogen, so it is necessary to screen all urinary isolates for biofilm production.

Keywords: Biofilm, Uropathogens, Tube adherence, Congo red agar.