“PREVALENCE OF BIOFILM FORMATION IN UROPATHOGEN’
Himanshu Trivedi, *Dr. Shweta Sao, Dr. Ramnesh Murthy and Dr. Sagrikapradhan
ABSTRACT
This study was conducted from February to may 2017 to determine the
prevalence, risk factors and Biofilm formation of selected bacterial
uropathogens among patients with urinary tract infection (UTI) cases in
CIMS Hospital, Bilaspur. A hospital-based cross-sectional survey was
conducted to assess the risk factors of UTI. In addition, laboratory
based work was conducted to determine the prevalence and bacterial
uropathogens using standard procedures. The isolates were identified
based on their morphological and biochemical characteristics.
Urinarytract infection (UTI) is the most commonly acquired bacterial
infection. The purpose of this study was to detect biofilm form ation by
uropathogenes isolated from UTIs. The isolated bacteria were tested for biofilm production by
tube adherence (TA), congoredagar (CRA) methods. Out of 195 urine specimen, 60 urine
samples (30.76%) are culture positive for uropathogen with significant colony count. These 60
uropathogen were processed in the present study for biofilm production, 36 (60%) were
culture positive and remaining 29 (48.33%) were culture negative. E. coli was the predominant
isolate 45/60 (75%) followed by Staphylococcus aureus 2/60 (3.33%), Klebsiella pneumonia
1/60(1.66%), Pseudomonas aeruginosa 2\60(3.33%), Coagulase negative Staphylococci
2\60(3.33%), Enterococcus faecali 4\60(6.66%), Proteus spp 2\60(3.33), Streptococcus spp
1\60(1.66%), Acinetoobacterspp 2\60(2.33%). The antibiotic resistance was higheramong
biofilm producers to commonly used antibiotics ascompared to nonbiofilm producers to its
detection by congoredagar method. From this study, we have concluded that TM and CRA
method is more qualitative and reliable method to detect biofilm producing microorganisms. TM and CRA method is used as a general screening method for detection of biofilm producing bacteria in laboratories. Biofilm form at ion is the major virulence determinant of uropathogen, so it is necessary to screen all urinary isolates for biofilm production.
Keywords: Biofilm, Uropathogens, Tube adherence, Congo red agar.
[Full Text Article]