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Muna H. Al-Salamy, *Mayyada F. Darweesh and AzharN. Almousawi


During the period from beginning of June 2014 tell end of December 2015, isolation and identification of S.lentus from end stage renal failure patients under haemodialysis therapy. A total of 400 clinical samples from 100 patients included (100 for each Skin, Urine, Blood, arterial Fistula, While 200 hospital environmental specimen included dialysis device (100), bed (100) from Nephrology department ALSadder Medical City in AL-Najaf province. Out of these samples, 50 isolates were confirmed as S.lentus by conventional bacteriological methods and vetik 2 compact system. Rest of these samples showed growth of other microorganism like as Klebsiella species, Staphylococcus species, E.coli etc. The antibiotic resistance of S.lentus bacteria has profound clinical implications. Hence, this research was aim for the first time in Iraq to isolation and study prevelance of S.lentus. From clinical and hospital environmental sources then determines their antimicrobial susceptibility patterns, the results revealed that S.lentus. isolated from Skin10 Urine 5, Blood 4 and arterial Fistula10. While 200 hospital environmental specimen included dialysis device12, bed 9. At the same time the results found that S.lentus were greatly resistant to antibiotics that most commonly used, so regard as multi-drug resistant (MDR), S.lentus isolates exhibited high resistance to Penicillin, Ceftazidime, Erythromycin, Clindamycin and Methicillin. While other antibiotics exhibited different activity against isolates. Furthermore, most isolates appeared low resistance to Tetracycline well Sulfamethoxazole / Trimthoprim and Levofloxacin while the isolates were sensitive to Vancomycin. Conclusion: The increase number of S.lentus isolated from humans indicates that S.lentus is an opportunistic pathogen and consider as one of nosocomial infection in Iraq and These organisms are found to be resistance to the routinely used antibiotics. Appropriate antimicrobial drugs should be prescribed after detected antibiogram. The patients should also be prevent stop taking the drugs in the middle. This will help in minimising the complications, and help in preventing the emergence of resistant strains.

Keywords: S.lentus, identification, haemodialysis, antibiotic sensitivity.

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