SPERM ACTIVATION OF OLIGOSPERMIC PATIENTS AT USING SWIM-DOWN TECHNIQUE AND SWIM-UP TECHNIQUE AFTER CENTERFUCATION BY FERTICULT FLUSHING MEDIUM WITH GLUTATHIONE
Dhirgam Falih Hassan*
ABSTRACT
This study was aimed to know effects of activation sperm for
Oligospermic infertile patients parameters by activated at using Swimdown
technique, Swim-up after centrifugation technique and using
FertiCult Flushing Medium with glutathione, This study was
performed on 44 samples, belongs to 30 patients suffering
oligospermia In this study divided one samples into two parts. The
first part activated by FertiCult Flushing Medium with glutathione and
Swim-down technique with incubation period 45 min in 37Co, the
second part activated by FertiCult Flushing Medium with glutathione
and Swim-up after centrifugation technique with incubation period
45min in 37Co. The results showed that there was a significant improvement (P<0.05) in the
sperm motility percent and sperm grade activity to that activation in Swim-down technique,
Swim-up after centrifugation technique comparison to the results before activation and the
results showed that there was a significant improvement (P>0.05) in the sperm concentration
and round cells concentration to that activation Swim-down technique, Swim-up after
centrifugation technique comparison to the results before activation and the results showed
that there was a significant improvement (P<0.05) in the normal sperm morphology percent
to that activation in Swim-up after centrifugation technique and Swim-down technique
comparison to the results before activation. Conclusion at using Swim-down technique, a
human sperm activation can give a better results on sperm concentration, sperm motility
percent and sperm grade activity in Oligospermia infertile patients parameters and using
FertiCult Flushing Medium with glutathione.
Keywords: Oligospermia, glutathione, swim-up and swim-down activation techniques.
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