Abstract

SPERM ACTIVATION OF OLIGOSPERMIC PATIENTS AT USING SWIM-DOWN TECHNIQUE AND SWIM-UP TECHNIQUE AFTER CENTERFUCATION BY FERTICULT FLUSHING MEDIUM WITH GLUTATHIONE

Dhirgam Falih Hassan*

ABSTRACT

This study was aimed to know effects of activation sperm for Oligospermic infertile patients parameters by activated at using Swimdown technique, Swim-up after centrifugation technique and using FertiCult Flushing Medium with glutathione, This study was performed on 44 samples, belongs to 30 patients suffering oligospermia In this study divided one samples into two parts. The first part activated by FertiCult Flushing Medium with glutathione and Swim-down technique with incubation period 45 min in 37Co, the second part activated by FertiCult Flushing Medium with glutathione and Swim-up after centrifugation technique with incubation period 45min in 37Co. The results showed that there was a significant improvement (P<0.05) in the sperm motility percent and sperm grade activity to that activation in Swim-down technique, Swim-up after centrifugation technique comparison to the results before activation and the results showed that there was a significant improvement (P>0.05) in the sperm concentration and round cells concentration to that activation Swim-down technique, Swim-up after centrifugation technique comparison to the results before activation and the results showed that there was a significant improvement (P<0.05) in the normal sperm morphology percent to that activation in Swim-up after centrifugation technique and Swim-down technique comparison to the results before activation. Conclusion at using Swim-down technique, a human sperm activation can give a better results on sperm concentration, sperm motility percent and sperm grade activity in Oligospermia infertile patients parameters and using FertiCult Flushing Medium with glutathione.

Keywords: Oligospermia, glutathione, swim-up and swim-down activation techniques.